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GrainGenes Reference Report: PRT-20-575

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Reference	PRT-20-575
Title	Interactions between genotypes and culture media components for improved in vitro response of rye (Secale cereale L.) inbred lines
Journal	Plant Cell Reports
Year	2001
Volume	20
Pages	575-582
Author	Popelka JC Altpeter F
Abstract	Summary: Rye is one of the most recalcitrant species in tissue culture. A multi-factorial experimental design allowed the development of genotype-specific tissue culture protocols, maximising the plant regeneration response from rye tissue culture. Precocious germination of explants, callus induction and callus maintenance (formation of rhizogenic and caulogenic callus) as well as regeneration response (percentage of regenerating calli and number of regenerated plants per callus) were significantly influenced by genotype, carbohydrate and auxin sources and their respective concentrations and interactions. Genotypes differed in the callus response to media sterilisation procedure, basic salt composition, gelling agent, CuSO4 complementation and illumination. These genotype-specific culture protocols will enable the development of efficient genetic transformation protocols for rye
Keyword	[ Hide all but 1 of 47 ] aestivum l calli anther culture auxin barley callus callus induction carbohydrate complementation component composition copper culture culture media design experimental design explants genetic transformation genotype genotype-culture medium interactions germination illumination immature embryo improved plant-regeneration in vitro in vitro system inbred lines induction inflorescences maintenance media oct plant regeneration protocol regenerated plants regeneration root regeneration rye rye (secale cereale l.) salt secale secale cereale somatic embryogenesis source tissue tissue culture transformation vitro

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