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GrainGenes Reference Report: PRT-20-67

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Reference	PRT-20-67
Title	Fertile transgenic plants obtained from tritordeum inflorescences by tissue electroporation
Journal	Plant Cell Reports
Year	2001
Volume	20
Pages	67-72
Author	He GY Lazzeri PA Cannell ME
Abstract	Summary: Tissue electroporation was applied to a member of the Triticeae family, namely tritordeum (Hordeum chilense Roem. x Triticum turgidum L. Conv. durum), for the generation of fertile transgenic plants. Two transgenic plants were recovered following the treatment of 361 explants of immature inflorescences (although they were subsequently found to result from the same transformation event). The expression of both inserted marker genes (uidA and bar) was confirmed using standard assays, while transgene integration was confirmed using PCR and Southern hybridization analyses. Integration pattern, segregation ratio and the inheritance of transgene expression in T1 progeny were consistent for the presence of a single transgene locus containing five to ten plasmid insertions. Although this procedure has been applied to other cereal species, stable transformation of the Triticeae using tissue electroporation has not previously been reported
Keyword	[ Hide all but 1 of 51 ] assay bar callus cereal dna delivery durum electroporation explants family fertile fertile transgenic plants gene gene transfer generation hordeum chilense hybridization immature inflorescences inflorescence inflorescences inheritance insertion integration locus maize marker marker genes member microprojectile bombardment pcr plasmid progeny protoplast rice plants seed embryo cells segregation single stable transformation tissue tissue electroporation transformation transgene transgene expression transgene inheritance transgene integration transgenic transgenic plant triticeae triticum turgidum tritordeum turgidum x triticum

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