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GrainGenes Reference Report: PRT-19-118

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Reference	PRT-19-118
Title	Analysis of particle bombardment parameters to optimise DNA delivery into wheat tissues
Journal	Plant Cell Reports
Year	1999
Volume	19
Pages	118-127
Author	Rasco-Gaunt S Riley A Barcelo P Lazzeri PA
Abstract	Summary: The objective of this study was to identify the major parameters controlling DNA delivery by particle bombardment to wheat (Triticum aestivum L.) scutellum and inflorescence tissue. The main factors studied were the DNA/gold precipitation process, bombardment parameters and tissue culture variables. Efficiency of DNA (uidA gene) delivery was assessed by scoring transient GUS expression in bombarded tissues. Of the parameters analysed, amount of plasmid DNA, spermidine concentration, presence of Ca(++) ions, calcium chloride concentration, amount of gold particles, gold particle size, acceleration pressure, chamber vacuum pressure, bombardment distance, osmotic conditioning of tissues and type of auxin had a clear influence on transient gene expression. A bombardment procedure suitable for elite wheat varieties was developed which allowed high-efficiency DNA delivery combined with reduced damage to target tissues
Keyword	[ Hide all but 1 of 28 ] auxin beta glucuronidase biolistics bombardment calcium calcium chloride calcium ions culture damage dna gene gene expression genetic transformation gus gus expression inflorescences particle bombardment particle size particles plasmid vectors reporter gene scutellum spermidine tissue tissue culture transgenic plant triticum aestivum wheat varieties

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