Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: PRT-18-924

[Submit comment/correction]

Reference	PRT-18-924
Title	Desiccated doubled-haploid embryos obtained from microspore culture of barley cv. Igri
Journal	Plant Cell Reports
Year	1999
Volume	18
Pages	924-928
Author	Ryan AB Castillo AM Valles MP Sanz JM Cistue L
Abstract	Summary: Barley microspore-derived doubled-haploid embryos have been produced in vitro. The development of embryo desiccation technology will allow long-term storage, germplasm preservation and low delivery cost. Treatment of the microspore-derived embryos was essential to induce desiccation tolerance and to arrest further development and plant regeneration. At the concentrations used, a treatment with trehalose was more efficient than with sucrose, and mannitol was harmful to the embryos. Up to 80% of the desiccated embryos produced complete green plants when transferred to regeneration medium, by the application of a 0.6 m trehalose or a 10(-5) m abscisic acid treatment to the embryos in the culture induction medium. The morphology of these plants was similar to plants produced directly from non-desiccated embryos
Keyword	[ Hide all but 1 of 22 ] abscisic acid anther culture barley cell culture culture media desiccation germplasm haploid hordeum vulgare in vitro induction mannitol morphology plant development plant embryos plant morphology pollen regrowth storage sucrose tolerance trehalose

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.