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GrainGenes Reference Report: PRT-15-301

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Reference	PRT-15-301
Title	Transgenic barley (Hordeum vulgare L.) by electroporation of protoplasts
Journal	Plant Cell Reports
Year	1995
Volume	15
Pages	301-304
Author	Salmenkallio-Marttila M [ Show all 8 ]
Abstract	Protoplasts isolated from calli derived from cultured microspores of barley (Hordeum vulgare L cv Kymppi, an elite cultivar) were transformed with the neomycin phosphotransferase marker gene (nptII) by electroporation Screening of the regenerated plants for the NPTII activity by gel assay resulted in three positive signals Southern blot analysis and NPTII assays of second and third generation plants confirmed the genomic integration of the transferred gene and that the new trait was inherited by the progeny
Keyword	[ Hide all but 1 of 17 ] anther-culture callus electroporation enzyme activity gene expression genes genetic markers genetic transformation hordeum vulgare inheritance neomycin nptii genes phosphotransferases pollen protoplast regenerative-ability transgenic-plants

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