Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: PRT-15-301

[Submit comment/correction]

Reference	PRT-15-301
Title	Transgenic barley (Hordeum vulgare L.) by electroporation of protoplasts
Journal	Plant Cell Reports
Year	1995
Volume	15
Pages	301-304
Author	[ Hide all but 1 of 8 ] Salmenkallio-Marttila M Aspegren K Akerman S Kurten U Mannonen L Ritala A Teeri T Kauppinen V
Abstract	Protoplasts isolated from calli derived from cultured microspores of barley (Hordeum vulgare L cv Kymppi, an elite cultivar) were transformed with the neomycin phosphotransferase marker gene (nptII) by electroporation Screening of the regenerated plants for the NPTII activity by gel assay resulted in three positive signals Southern blot analysis and NPTII assays of second and third generation plants confirmed the genomic integration of the transferred gene and that the new trait was inherited by the progeny
Keyword	anther-culture [ Show all 17 ]

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.