Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: PRT-15-232

[Submit comment/correction]

Reference	PRT-15-232
Title	Factors affecting the establishment and maintenance of embryogenic callus and suspension cultures of wheat (Triticum aestivum L.)
Journal	Plant Cell Reports
Year	1995
Volume	15
Pages	232-237
Author	Fellers J Guenzi A Taliaferro C
Abstract	Improved suspension cell culture systems are needed to facilitate the application of recombinant DNA technology for wheat germplasm enhancement This study evaluated three wheat (Triticum aestivum L. cultivars, and the effects of medium basal salts, 2,4-D, sucrose, and L-proline concentrations on the establishment of rapidly growing and highly embryogenic callus and suspension cultures Percent embryogenic calli was visually estimated and verified with light and scanning electron microscopy. The most highly embryogenic callus was produced by cultivar Bobwhite on medium with MS basal salts, 5.6 micromolars 2,4-D, 58 mM sucrose, and zero proline. The suspension cultures that produced the greatest number of regenerated plants utilized callus tissue produced on solid medium with MS basal salts, 87 mM sucrose, 9 micromolars 2,4-D, and no proline.
Keyword	[ Hide all but 1 of 30 ] 2 4-d basal-salts callus cell-growth cell-suspensions cultivar culture-media dna dosage-effects embryogenesis genetic variation germplasm growth stage growth-rate iaa in-vitro-culture methodology murashige-and-skoog-medium naa plant anatomy proline quantitative-analysis recombination redway-and-vasil-medium salts sucrose triticum aestivum ultrastructure zea in

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.