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GrainGenes Reference Report: PRT-15-186

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Reference	PRT-15-186
Title	In vitro development of globular zygotic wheat embryos
Journal	Plant Cell Reports
Year	1995
Volume	15
Pages	186-191
Author	Fischer C Neuhaus G
Abstract	We have established in vitro culture conditions for globular zygotic wheat embryos (Triticum aestivum L. Their nutritional requirements have been systematically investigated. The initial sucrose concentration, as well as the sucrose concentration during the culture, a 6-benzylaminopurine supplement, the use of nitrates and ammonium as nitrogen source have a major influence on the embryo development. Proline has an inhibitory effect on the germination. A double layer system with different media was used to give a continuous variation of the medium composition with time. These culture conditions allowed normal direct embryogenesis in up to 47% of the globular embryos.
Keyword	[ Hide all but 1 of 20 ] 2-n-morpholinoethane-sulfonic-acid ammonium benzyladenine cell-differentiation cell-growth culture-media developmental-stages dosage-effects embryogenesis germination-inhibitors glutamine nitrates nitrogen nutrient-sources plant embryos plant morphology proline sucrose triticum aestivum zygotes

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