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GrainGenes Reference Report: PRT-14-437

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Reference	PRT-14-437
Title	Effect of 2,4-D precultivation on regeneration capacity of cultivated barley
Journal	Plant Cell Reports
Year	1995
Volume	14
Pages	437-441
Author	Vitanova Z Vitanov V Trifonova A Savova D Atanassov A
Abstract	A reliable protocol for regeneration of barley from seedling explants including leaf bases with the complete apical meristem has been developed Callus induction and shoot regeneration was only achieved when 28 days-old-seedlings were grown in the presence of high concentrations of 2,4-D The Bulgarian barley variety Ruen commercially important for brewery industry was used as a model The regeneration response of a range of Bulgarian barley genotypes was evaluated Cytological analyses were performed at the level of callus and regenerants
Keyword	[ Hide all but 1 of 18 ] 2 4-d apical-meristems bulgaria callus cultivar culture-media cytology explants genetic variation genotype hordeum vulgare in-vitro-culture leaves ploidy pretreatment regenerative-ability seedling

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