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GrainGenes Reference Report: PRT-14-354

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Reference	PRT-14-354
Title	Oat leaf base: tissue with an efficient regeneration capacity
Journal	Plant Cell Reports
Year	1995
Volume	14
Pages	354-358
Author	Chen Z Zhuge Q Sundqvist C
Abstract	An efficient short term regeneration system using seedling derived oat (Avena sativa) leaf tissue has been developed Callus derived from the leaf base showed a higher response of plant regeneration than callus initiated from mesocotyls and more mature parts of the leaves A correlation between the nuclear DNA content of the donor material, as analysed with flow cytometry, and its ability to form callus was observed Somatic embryogenesis was histologically recognised from callus derived from tissue close to the apical meristem Plant regeneration media with various concentrations of auxin were tested Callus from three different cultivars had a similar regeneration potential with an optimal regeneration frequency of 60% About 2 months after inoculation regenerated plantlets could be moved to a greenhouse for cultivation
Keyword	[ Hide all but 1 of 24 ] 2,4-d 6-diamideno-2-phenylidole apical-meristems avena sativa callus cultivar culture-media culture-techniques dna abt explants genetic analysis histology hypocotyls iaa indoles kinetin leaves mesocotyls naa plant anatomy plant morphology quantitative-analysis regenerative-ability ultrastructure

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