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GrainGenes Reference Report: PRT-14-305

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Reference	PRT-14-305
Title	Production of transgenic sugarcane (Saccharum officinarum L.) plants by intact cell electroporation
Journal	Plant Cell Reports
Year	1995
Volume	14
Pages	305-309
Author	Arencibia A Molina P Riva G Selman-Housein G
Abstract	We describe an efficient procedure for genetic transformation of commercial sugarcane varieties POJ 2878 and Ja 60-5 The transformation protocol is based on electroporation of a plasmid conferring GUS activity into cell clusters isolated from embryogenic calli Six to eight weeks after electroporation, Ja 60-5 plants regenerated from electroporated tissues were tested and confirmed to be transgenic using histochemical glucuronidase and Southern hybridization analysis Electroporation of intact cells is an efficient and reproducible method for sugarcane transformation and may also be useful for transformation of other plants
Keyword	[ Hide all but 1 of 14 ] beta-glucuronidase callus cell culture electroporation embryogenesis enzyme activity gene expression genetic transformation histochemistry plant breeding methods plasmids saccharum officinarum transgenic plants varieties

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