GrainGenes Reference Report: PRT-13-489
Reference
PRT-13-489
Title
A thin layer chromatographic technique for detecting inducers of Agrobacterium virulence genes in corn, wheat and rye
Journal
Plant Cell Reports
Year
1994
Volume
13
Pages
489-492
Author
Sahi S Gagliardo R Chilton M Chilton W
Abstract
A method is described for detecting plant metabolites capable of inducing the virulence genes of Agrobacterium tumefaciens The method uses A tumefaciens containing a plasmid with an inducible virulence gene fused to a galactosidase gene (virE::lacZ) Thin layer chromatography plates are overlayed with agar containing the indicator bacterium and a chromogenic galactoside (X-gal) Virulence gene inducing plant metabolites induce galactosidase which releases an aglycone readily oxidized by air to a blue pigmented zone at the Rf of the inducer The method has been used to demonstrate the presence of virulence gene inducers in corn, wheat and rye The uninduced background level of galactosidase also permits detection of bacterial growth inhibitors after a longer incubation period.
Keyword
[ Hide all but 1 of 11 ] agrobacterium-tumefaciens chemical-analysis galactosidases genes induction metabolites plant extracts secale cereale triticum monococcum virulence zea mays
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GrainGenes Reference Report: PRT-13-489
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||