GrainGenes Reference Report: PRT-12-506
Reference
PRT-12-506
Title
The effect of different promoter-sequences on transient expression of gus reporter gene in cultured barley (Hordeum vulgare L.) cells
Journal
Plant Cell Reports
Year
1993
Volume
12
Pages
506-509
Author
Chibbar R
[ Show all 7 ]
Abstract
The cauliflower mosaic virus 35S (35S) and the enhanced 35S (E35S) promoters fused with maize alcoholdehydrogenase (Adh1) intron1 or maize shrunken locus (sh1) intron1 along with maize Adh1 and rice actin (Act1) promoters fused to their respective first introns were tested for transient expression of the E.coli beta- glucuronidase (gus) reporter gene in cultured barley (Hordeum vulgare L) cells. The plasmids, carrying the respective promoter- intron combinations to drive the gus fused to nopaline synthase (nos) terminator, were introduced into cultured barley cells using a particle gun. The rice Act1 promoter with its first intron gave the highest expression of all promoter intron combinations studied. This was followed by the E35S promoter and no significant differences were observed between the other two promoters tested. The rice actin promoter is now being used to drive selectable marker genes to obtain stably transformed cereal cells.
Keyword
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: PRT-12-506
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||