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GrainGenes Reference Report: PRT-12-435

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Reference	PRT-12-435
Title	Stable transformation of barley tissue culture by particle bombardment
Journal	Plant Cell Reports
Year	1993
Volume	12
Pages	435-440
Author	Ritala A [ Show all 9 ]
Abstract	Suspension culture cells of barley (Hordeum vulgare L cv Pokko) were stably transformed with two separate plasmids containing genes coding for neomycin phosphotransferase II and beta-glucuronidase, respectively Transformed cultures were selected with the antibiotic Geneticin(R) Enzymatic activity was tested in the Geneticin(R) resistant cultures, and in 96% of them neomycin phosphotransferase could be detected The non-selected marker, detected as beta-glucuronidase activity, was expressed in 40% of the resistant cultures Stable transformation was confirmed with Southern blot hybridization
Keyword	[ Hide all but 1 of 18 ] antibiotics beta-glucuronidase cell-suspensions culture-media enzyme activity gene expression gene transfer genetic code genetic markers genetic n genetic transformation hordeum vulgare neomycin pat13 phosphotransferases phtt303 plasmids selection

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