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GrainGenes Reference Report: PPS-136-2747

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Reference	PPS-136-2747
Title	Two transcription factors are negative regulators of gibberellin response in the HvSPY-signaling pathway in barley aleurone
Journal	Plant Physiology
Year	2004
Volume	136
Pages	2747-2761
Author	Robertson M
Abstract	spindly (spy) protein from barley (hordeum vulgare cv. himalaya; hvspy) negatively regulated ga responses in aleurone, and genetic analyses of arabidopsis thaliana predict that spy functions in a derepressible ga-signalling pathway. many, if not all, ga-dependent responses require spy protein, and to improve our understanding of how the spy signalling pathway operates, a yeast two-hybrid screen was used to identify both upstream and downstream components that might regulate the activity of the hvspy protein. a number of proteins from diverse classes were identified using hvspy as bait and barley cdna libraries as prey. two of the hvspy-interacting (hsi) proteins were transcription factors belonging to the myb and nac gene families, hsimyb and hsinac. interaction occurred via the tetratricopeptide repeat domain of hvspy and specificity was shown both in vivo and in vitro. messenger rnas for these proteins were expressed differentially in many parts of the barley plant but at very low levels. both hsimyb and hsinac inhibited the ga3 upregulation of alpha -amylase expression in aleurone, both were activators of transcription in yeast, and the green fluorescent protein-hsi fusion proteins were localized in the nucleus. these results are consistent with the model that hsi transcription factors act downstream of hvspy as negative regulators and that they in turn could activate other negative regulators, forming the hvspy negative regulator-signalling pathway for ga response
External Databases	Pubmed: 15347799
Keyword	[ Hide all but 1 of 21 ] aleurone cells alpha-amylase barley biochemical pathways complementary dna dna libraries enzyme gene expression genetic analysis gibberellic acid gibberellins messenger rna nuclei plant growth regulators plant proteins protein rna signal transduction transactivation transcription transcription factor

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