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GrainGenes Reference Report: PPS-131-558

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Reference	PPS-131-558
Title	Powdery mildew-induced Mla mRNAs are alternatively spliced and contain multiple upstream open reading frames
Journal	Plant Physiology
Year	2003
Volume	131
Pages	558-567
Author	Halterman DA Wei FS Wise RP
Abstract	Summary: In barley (Hordeum vulgare), the Mla13 powdery mildew resistance gene confers Rar1-dependent, AvrMla13-specific resistance to Blumeria graminis f. sp. hordei (Bgh). We have identified cDNA and genomic copies of Mla13 and used this coiled-coil nucleotide-binding site leucine-rich repeat protein-encoding gene as a model for the regulation of host resistance to obligate biotrophic fungi in cereals. We demonstrate quantitatively that a rapid increase in the accumulation of Mla transcripts and transcripts of the Mla-signaling genes, Rar1 and Sgt1, is triggered between 16 and 20 h post inoculation, the same time frame that haustoria of avirulent Bgh make contact with the host cell plasma membrane. An abundance of Mla13 cDNAs revealed five classes of transcript leader regions containing two alternatively spliced introns and up to three upstream open reading frames (uORFs). Alternative splicing of introns in the transcript leader region results in a different number of uORFs and variability in the size of uORF2. These results indicate that regulation of Mla transcript accumulation is not constitutive and that induction is coordinately controlled by recognition-specific factors. The sudden increase in specific transcript levels could account for the rapid defense response phenotype conferred by Mla6 and Mla13
External Databases	Pubmed: 12586880
Gene	Mla13 (Hordeum)
Sequence	AF523683
Keyword	[ Hide all but 1 of 45 ] alternative splicing barley blumeria graminis cdna cell cell death defense defense response erysiphe graminis f-sp hordei flax rust resistance fungi gene graminis haustoria hordei hordeum increase induction inoculation intron introns membrane mildew mildew resistance model mosaic-virus resistance multiple nucleotide binding nucleotide binding site plant disease resistance plasma membrane powdery mildew powdery mildew resistance region regulation repeat resistance resistance gene rich repeat class site transcript transcript accumulation transcript levels variability

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