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GrainGenes Reference Report: PPS-130-1706

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Reference	PPS-130-1706
Title	Expressed sequence tag-based gene expression analysis under aluminum stress in Rye
Journal	Plant Physiology
Year	2002
Volume	130
Pages	1706-1716
Author	Milla MAR [ Show all 6 ]
Abstract	Summary: To understand the mechanisms responsible for aluminum (Al) toxicity and tolerance in plants, an expressed sequence tag (EST) approach was used to analyze changes in gene expression in roots of rye (Secale cereale L. cv Blanco) under Al stress. Two cDNA libraries were constructed (Al stressed and unstressed), and a total of 1,194 and 774 ESTs were generated, respectively. The putative proteins encoded by these cDNAs were uncovered by Basic Local Alignment Search Tool searches, and those ESTs showing similarity to proteins of known function were classified according to 13 different functional categories. A total of 671 known function genes were used to analyze the gene expression patterns in rye cv Blanco root tips under Al stress. Many of the previously identified Al-responsive genes showed expression differences between the libraries within 6 h of Al stress. Certain genes were selected, and their expression profiles were studied during a 48-h period using northern analysis. A total of 13 novel genes involved in cell elongation and division (tonoplast aquaporin and ubiquitin-like protein SMT3), oxidative stress (glutathione peroxidase, glucose-6-phosphate-dehydrogenase, and ascorbate peroxidase), iron metabolism (iron deficiency-specific proteins IDS3a, IDS3b, and IDS1; S-adenosyl methionine synthase; and methionine synthase), and other cellular mechanisms (pathogenesis-related protein 1.2, heme oxygenase, and epoxide hydrolase) were demonstrated to be regulated by Al stress. These genes provide new insights about the response of Al-tolerant plants to toxic levels of Al
External Databases	Pubmed: 12481053
Keyword	[ Hide all but 1 of 34 ] alignment aluminum ascorbate peroxidase barley roots cdna cell cell elongation cloning elongation est gene glutathione heme heme oxygenase iron metabolism methionine oxidative stress oxygenase pathogenesis-related protein peroxidase rye secale secale cereale sequence similarities stress synthase tobacco cells tolerance tonoplast tool toxicity wheat triticum-aestivum

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