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GrainGenes Reference Report: PPS-130-362

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Reference	PPS-130-362
Title	Tandemly duplicated safener-induced glutathione S-transferase genes from Triticum tauschii contribute to genome- and organ-specific expression in hexaploid wheat
Journal	Plant Physiology
Year	2002
Volume	130
Pages	362-373
Author	Xu FX Lagudah ES Moose SP Riechers DE
Abstract	Summary: Glutathione S-transferase (GST) gene expression was examined in several Triticum species, differing in genome constitution and ploidy level, to determine genome contribution to GST expression in cultivated, hexaploid bread wheat (Triticum aestivum). Two tandemly duplicated tau class GST genes (TtGSTU1 and TtGSTU2) were isolated from a single bacterial artificial chromosome clone in a library constructed from the diploid wheat and D genome progenitor to cultivated wheat, Triticum tauschii. The genes are very similar in genomic structure and their encoded proteins are 95% identical. Gene-specific reverse transcriptase-polymerase chain reaction analysis revealed differential transcript accumulation of TtGSTU1 and TtGSTU2 in roots and shoots. Expression of both genes was induced by herbicide safeners, 2,4-dichlorophenoxyacetic acid and abscisic acid, in the shoots of T. tauschii; however, expression of TtGSTU1 was always higher than TtGSTU2. In untreated seedlings, TtGSTU1 was expressed in both shoots and roots, whereas TtGSTU2 expression was only detected in roots. RNA gel-blot analysis of ditelosomic, aneuploid lines that are deficient for 6AS, 6BS, or 6DS chromosome arms of cultivated, hexaploid bread wheat showed differential genome contribution to safener-induced GST expression in shoots compared with roots. The GST genes from the D genome of hexaploid wheat contribute most to safener-induced expression in the shoots, whereas GSTs from the B and D genomes contribute to safener-induced expression in the roots
External Databases	Pubmed: 12226515
Keyword	[ Hide all but 1 of 48 ] 2,4-dichlorophenoxyacetic acid acid aneuploid aneuploid lines arms artificial chromosome bacterial bacterial artificial chromosome binding protein bread chain chromosome chromosome arm clone contribute deficient diploid diploid wheat ditelosomic encoded proteins gene genomic structure glutathione glutathione-s-transferase gst1 gene herbicide herbicide safener hexaploid hexaploid bread wheat hordeum vulgare hydrogen peroxide isoform-ii maize zea-mays ocs element organ-specific expression ploidy progenitor responsive enhancer element s-transferase seedling shoot structure tauschii transcript triticum triticum aestivum triticum tauschii wide-range

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