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GrainGenes Reference Report: PPS-129-1308

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Reference	PPS-129-1308
Title	Proteome analysis of grain filling and seed maturation in barley
Journal	Plant Physiology
Year	2002
Volume	129
Pages	1308-1319
Author	Finnie C Melchior S Roepstorff P Svensson B
Abstract	Summary: In monocotyledonous plants, the process of seed development involves the deposition of reserves in the starchy endosperm and development of the embryo and aleurone layer. The final stages of seed development are accompanied by an increase in desiccation tolerance and drying out of the mature seed. We have used two-dimensional gel electrophoresis for a time-resolved study of the changes in proteins that occur during seed development in barley (Hordeum vulgare). About 1,000 low-salt extractable protein spots could be resolved on the two-dimensional gels. Protein spots were divided into six categories according to the timing of appearance or disappearance during the 5-week period of comparison. Nineteen different proteins or protein fragments in 36 selected spots were identified by matrix-assisted laser-desorption ionization time of flight mass spectrometry (MS) or nano-electrospray tandem MS/MS. Some proteins were present throughout development (for example, cytosolic malate dehydrogenase), whereas others were associated with the early grain filling (ascorbate peroxidase) or desiccation (Cor14b) stages. Most noticeably, the development process is characterized by an accumulation of low-Mr alpha-amylase/trypsin inhibitors, serine protease inhibitors, and enzymes involved in protection against oxidative stress. We present examples of proteins not previously experimentally observed, differential extractability of thiol-bound proteins, and possible allele-specific spot variation. Our results both confirm and expand on knowledge gained from previous analyses of individual proteins involved in grain filling and maturation
Keyword	[ Hide all but 1 of 63 ] 2-dimensional electrophoresis aleurone aleurone layer appearance ascorbate ascorbate peroxidase barley dehydrogenase deposition desiccation desiccation tolerance drying electrophoresis embryo endosperm endosperm development extractability fragments gel-electrophoresis gels gene duplication glyoxalase-i hordeum immobilized ph gradients ionization knowledge laser-desorption ionization laser-desorption-ionization layer malate malate-dehydrogenase mass spectrometry mass-spectrometry matrix assisted laser desorption maturation monocotyledonous plant monocotyledonous plants oxidative stress oxidative-stress peptide mass fingerprints peroxidase polyacrylamide-gel electrophoresis protease protease inhibitor protease inhibitors protection proteome proteome analysis seed seed development seed maturation serine serine protease spectrometry spot starchy endosperm stress stresses tandem tolerance two-dimensional electrophoresis two-dimensional gel electrophoresis wheat-grain

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