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GrainGenes Reference Report: PPS-129-1265

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Reference	PPS-129-1265
Title	Glyphosate-resistant goosegrass. Identification of a mutation in the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase
Journal	Plant Physiology
Year	2002
Volume	129
Pages	1265-1275
Author	Baerson SR [ Show all 6 ]
Abstract	Summary: The spontaneous occurrence of resistance to the herbicide glyphosate in weed species has been an extremely infrequent event, despite over 20 years of extensive use. Recently, a glyphosate-resistant biotype of goosegrass (Eleusine indica) was identified in Malaysia exhibiting an LD50 value approximately 2- to 4-fold greater than the sensitive biotype collected from the same region. A comparison of the inhibition of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) activity by glyphosate in extracts prepared from the resistant (R) and sensitive (S) biotypes revealed an approximately 5-fold higher IC50(glyphosate) for the (R) biotype. Sequence comparisons of the predicted EPSPS mature protein coding regions from both biotypes revealed four single-nucleotide differences, two of which result in amino acid changes. One of these changes, a proline to serine substitution at position 106 in the (R) biotype, corresponds to a substitution previously identified in a glyphosate-insensitive EPSPS enzyme from Salmonella typhimurium. Kinetic data generated for the recombinant enzymes suggests that the second substitution identified in the (R) EPSPS does not contribute significantly to its reduced glyphosate sensitivity. Escherichia coli aroA (EPSPS deficient) strains expressing the mature EPSPS enzyme from the (R) biotype exhibited an approximately 3-fold increase in glyphosate tolerance relative to strains expressing the mature EPSPS from the (S) biotype. These results provide the first evidence for an altered EPSPS enzyme as an underlying component of evolved glyphosate resistance in any plant species
Keyword	[ Hide all but 1 of 52 ] 5 enolpyruvylshikimate 3 phosphate synthase 5-enolpyruvylshikimate-3-phosphate synthase alkyl (aryl) transferases amino acid amino acid sequence amino acid substitution biotype biotypes cell growth coding region complementary dna deficient difference eleusine indica enzyme enzyme activity epsps escherichia coli gene expression genetic variation genome glyphosate herbicide herbicide glyphosate herbicide resistance indica inhibition kinetics malaysia messenger rna molecular sequence data nucleotide sequence plant composition plant proteins plant species position proline protein recombinant enzymes resistance resistant salmonella sensitivity sequence sequence comparisons serine substitution synthase target tolerance weed weed control

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