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GrainGenes Reference Report: PPS-127-937

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Reference	PPS-127-937
Title	The Arabidopsis protein SHI represses gibberellin responses in Arabidopsis and barley
Journal	Plant Physiology
Year	2001
Volume	127
Pages	937-948
Author	Fridborg I Kuusk S Robertson M Sundberg E
Abstract	Summary: The current model of gibberellin (GA) signal transduction is based on a derepressible system and a number of candidate negative regulators have been identified in Arabidopsis. We previously have reported the identification of the Arabidopsis gene SHORT INTERNODES (SHI) that causes suppression of GA responses when constitutively activated. In this paper, we show by using reporter gene analysis that the SHI gene is expressed in young organs, e.g. shoot apices and root tips. The model predicts a suppressor of GA responses to be active in these tissues to prevent premature growth or development. To study the effect of SHI on GA signaling, we used a functional assay that measures effects of signaling components on a well-defined GA response; the up-regulation of alpha-amylase in barley (Hordeum vulgare) aleurones in response to GA treatment. We found that SHI was able to specifically block the activity of a high-isoelectric point alpha-amylase promoter following GA3 treatment, which further supports that SHI is a suppressor of GA responses. We have identified two putative loss-of-function insertion alleles of SHI and lines homozygous for either of the new alleles show no phenotypic deviations from wild type. Because SHI belongs to a gene family consisting of nine members, we suggest that SHI and the SHI-related genes are functionally redundant. We also show that a functional ERECTA allele is able to partly suppress the dwarfing effect of the shi gain-of-function mutation, suggesting that the erecta mutation harbored by the Landsberg erecta ecotype is an enhancer of the shi dwarf phenotype
External Databases	Pubmed: 11706176
Keyword	[ Hide all but 1 of 58 ] abscisic acid aleurone allele alleles arabidopsis arabidopsis gene assay barley belongs component dwarf dwarfing enhancer family ga ga response gain-of-function gene gene encodes gene family gibberellin growth homozygous hordeum hordeum vulgare insertion internodes loss-of-function member model mutant mutation negative regulator paper phenotype promoter protein regulator regulators reporter reporter gene responses root root tips shoot shoot apices signal signal transduction supports suppression suppressor system thaliana l heynh tissue transduction wild wild-type zinc-finger

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