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GrainGenes Reference Report: PPS-125-1216

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Reference	PPS-125-1216
Title	A complete set of maize individual chromosome additions to the oat genome
Journal	Plant Physiology
Year	2001
Volume	125
Pages	1216-1227
Author	Kynast RG [ Show all 14 ]
Abstract	Summary: All 10 chromosomes of maize (Zea mays, 2n = 2x = 20) were recovered as single additions to the haploid complement of oat (Avena sativa, 2n = 6x = 42) among F1 plants generated from crosses involving three different lines of maize to eight different lines of oat. In vitro rescue culture of more than 4,300 immature F1 embryos resulted in a germination frequency of 11% with recovery of 379 F1 plantlets (8.7%) of moderately vigorous growth. Some F1 plants were sectored with distinct chromosome constitutions among tillers of the same plant and also between root and shoot cells. Meiotic restitution facilitated development of un-reduced gametes in the F1. Self-pollination of these partially fertile F1 plants resulted in disomic additions (2n = 6x + 2 = 44) for maize chromosomes 1, 2, 3, 4, 6, 7, and 9. Maize chromosome 8 was recovered as a monosomic addition (2n = 6x + 1 = 43). Monosomic additions for maize chromosomes 5 and 10 to a haploid complement of oat (n = 3x + 1 = 22) were recovered several times among the F1 plants. Although partially fertile, these chromosome 5 and 10 addition plants have not yet transmitted the added maize chromosome to F2 offspring. We discuss the development and general utility of this set of oat-maize addition lines as a novel tool for maize genomics and genetics
External Databases	Pubmed: 11244103
Keyword	[ Hide all but 1 of 42 ] addition lines avena avena sativa cell chromosome chromosome addition constitutions cross culture development embryo fertile frequencies gametes genome germination growth haploid haploid production frequencies hexaploid wheat hordeum bulbosum in vitro line maize mays monosomic additions n oat oat-maize addition line rescue root self-pollination shoot single tillers tool triticum aestivum unreduced gamete wide hybridization x maize zea zea mays

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