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GrainGenes Reference Report: PPS-122-657

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Reference	PPS-122-657
Title	Expression of aluminum-induced genes in transgenic Arabidopsis plants can ameliorate aluminum stress and/or oxidative stress
Journal	Plant Physiology
Year	2000
Volume	122
Pages	657-665
Author	Ezaki B Gardner RC Ezaki Y Matsumoto H
Abstract	Summary: To examine the biological role of Al-stress-induced genes, nine genes derived from Arabidopsis, tobacco (Nicotiana tabacum L.), wheat (Triticum aestivum L.), and yeast (Saccharomyces cerevisiae) were expressed in Arabidopsis ecotype Landsberg. Lines containing eight of these genes were phenotypically normal and were tested in root elongation assays for their sensitivity to Al, Cd, Cu, Na, Zn, and to oxidative stresses. An Arabidopsis blue-copper-binding protein gene (AtBCB), a tobacco glutathione S-transferase gene (parB), a tobacco peroxidase gene (NtPox), and a tobacco GDP-dissociation inhibitor gene (NtGDI1) conferred a degree of resistance to Al. Two of these genes, AtBCB and parB, and a peroxidase gene from Arabidopsis (AtPox) also showed increased resistance to oxidative stress induced by diamide, while parB conferred resistance to Cu and Na. Al content of Al-treated root tips was reduced in the four Al-resistant plant lines compared with wild-type Ler-0, as judged by morin staining. All four Al-resistant lines also showed reduced staining of roots with 2',7'-dichloro fluorescein diacetate (H(2)DCFDA), an indicator of oxidative stress. We conclude that Al-induced genes can serve to protect against Al toxicity, and also provide genetic evidence for a link between Al stress and oxidative stress in plants
External Databases	Pubmed: 10712528
Keyword	[ Hide all but 1 of 53 ] al aluminum arabidopsis arabidopsis thaliana assay bioassay cadmium cd copper cu cultured tobacco cells elongation fluorescein gene genetic regulation glutathione glutathione transferase glutathione-s-transferase growth guanosine diphosphate heat-shock factor inhibitor metal tolerance nicotiana nicotiana tabacum oxidation oxidative stress peroxidase phenotypes phytotoxicity plant proteins protein gene resistance root root tips roots saccharomyces cerevisiae sensitive mutants sensitivity sodium stress superoxide-dismutase tobacco tolerance toxicity transgenic transgenic arabidopsis transgenic plant triticum aestivum wheat roots yeast zinc zn

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