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GrainGenes Reference Report: PPS-118-1147

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Reference	PPS-118-1147
Title	Characterization of a low-molecular weight glutenin subunit gene from bread wheat and the corresponding protein that represents a major subunit of the glutenin polymer.
Journal	Plant Physiology
Year	1998
Volume	118
Pages	1147-1158
Author	Masci S D'Ovidio R Lafiandra D Kasarda DD
Abstract	Both high- and low-molecular weight glutenin subunits (LMW-GS) play the major role in determining the viscoelastic properties of wheat (Triticum aestivum L.) flour. To date there has been no clear correspondence between the amino acid sequences of LMW-GS derived from DNA sequencing and those of actual LMW-GS present in the endosperm. We have characterized a particular LMW-GS from hexaploid bread wheat, a major component of the glutenin polymer, which we call the 42K LMW-GS, and have isolated and sequenced the putative corresponding gene. Extensive amino acid sequences obtained directly for this 42K LMW-GS indicate correspondence between this protein and the putative corresponding gene. This subunit did not show a cysteine (Cys) at position 5, in contrast to what has frequently been reported for nucleotide-based sequences of LMW-GS. This Cys has been replaced by one occurring in the repeated-sequence domain, leaving the total number of Cys residues in the molecule the same as in various other LMW-GS. On the basis of the deduced amino acid sequence and literature-based assignment of disulfide linkages, a computer-generated molecular model of the 42K subunit was constructed.
External Databases	Pubmed: 9847089 Agricola: 2004-33060
Keyword	[ Hide all but 1 of 43 ] amino acid amino acid sequence analogs and derivatives biopolymers bread bread wheat chemical structure cloning computer simulation cysteine dna dna sequencing domain endosperm flour food composition gene genes genetic regulation gluten glutenin glutenin subunit glutenins hexaploid linkage lmw gs low-molecular-weight glutenin subunits model models,molecular molecular sequence data molecular weight peptide fragments polymer polymerase chain reaction protein composition protein conformation purification sequence triticum triticum aestivum viscoelastic properties viscoelasticity wheat flour

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