Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: PPS-104-639

[Submit comment/correction]

Reference	PPS-104-639
Title	Characterization of a family of chlorophyll-deficient wheat (Triticum) and barley (Hordeum vulgare) mutants with defects in the magnesium-insertion step of chlorophyll biosynthesis
Journal	Plant Physiology
Year	1994
Volume	104
Pages	639-648
Author	Falbel T Staehelin L
Abstract	During thylakoid membrane biogenesis, chlorophyll (Chl) biosynthesis and the accumulation of Chl-binding proteins are tightly linked, light-regulated processes We have investigated the consequences faced by mutant plants with defects in Chl biosynthesis by studying a series of five homeologous allelic chlorina mutants in wheat (Triticum) and one phenotypically related barley (Hordeum vulgare) mutant that express the same pleiotropic mutant phenotype but to different extents These mutants accumulate Chl at different rates, with the most severely affected plants having slowest rate of Chl accumulation Analysis of precursor p( the Chl synthesis pathway indicates they have a partial block in Chl synthesis and accumulate protoporphyrin IX (Proto), the last porphyrin compound common to both heme and Chl synthesis The affected plants with the most severe phenotypes accumulate the most Proto Chloroplasts isolated from these mutants exhibit a lower activity of the enzyme Mg-chelatase, which catalyzes the first committed step in Chl synthesis The most severely affected plants exhibit the greatest reduction in Mg-chelatase activity Heme levels and protoporphyrinogen oxidase activity were the same for mutant and wild-type plants. We suggest that a block in Mg-chelatase activity in these mutants could account for the other traits of their pleiotropic phenotype previously described in the literature.
Keyword	[ Hide all but 1 of 22 ] alleles b-binding-protein biochemical-pathways biosynthesis chelating-agents chlorophyll chlorophyll-a deficiency enzyme enzyme activity ferrochelatase heme hordeum vulgare magnesium mg-chelatase mutant oxidoreductases phenotype porphyrins precursors thylakoids triticum aestivum

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.