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GrainGenes Reference Report: PPS-100-1780

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Reference	PPS-100-1780
Title	The effect of heat shock on morphogenesis in barley. Coordinated circadian regulation of mRNA levels for light-regulated genes and of the capacity for accumulation of chlorophyll protein complexes
Journal	Plant Physiology
Year	1992
Volume	100
Pages	1780-1786
Author	Beator J Potter E Kloppstech K
Abstract	The effect of daily heat-shock treatments on gene expression and morphogenesis of etiolated barley (Hordeum vulgare) was investigated Heat-shock treatments in the dark induced shortening of the primary leaves and the coleoptiles to the length of those in light-grown plantlets In addition, the mRNA levels of the light-induced genes that were investigated were raised under these conditions and showed distinct oscillations over a period of at least 3 d While the mRNA levels for chlorophyll alb binding protein (LHC II), plastocyanin, and the small subunit of ribulose-1,5-bisphosphate carboxylase had maxima between 8 and 12 Pm (12-16 h after the last heat-shock treatment), the mRNA levels for thionin oscillated with a phase opposed to that of LHC II Etiolated barley, the circadian oscillator of which was synchronized by cyclic heat-shock treatments, was illuminated for a constant interval at different times of the day; this led to the finding that greening was fastest at the time when the maximal levels of mRNA for LHC II were also observed Whereas accumulation of chlorophyll a during a 4-h period of illumination oscillated by a factor of 3, chlorophyll b accumulation changed 10- to 15-fold Similarly, accumulation of LHC II was highest when pigments accumulated maximally Hence, greening or, in other words, thylakoid membrane assembly is under control of the circadian oscillator. expression and morphogenesis of etiolated barley (Hordeum vulgare) was investigated. Heat-shock treatments in the dark induced shortening of the primary leaves and the coleoptiles to the length of those in light-grown plantlets. In addition, the mRNA levels of the light-induced genes that were investigated were raised under these conditions and showed distinct oscillations over a period of at least 3 d. While the mRNA levels for chlorophyll alb binding protein (LHC II), plastocyanin, and the small subunit of ribulose-1,5-bisphosphate carboxylase had maxima between 8 and 12 PM (12-16 h after the last heat-shock treatment), the mRNA levels for thionin oscillated with a phase opposed to that of LHC II. Etiolated barley, the circadian oscillator of which was synchronized by cyclic heat-shock treatments, was illuminated for a constant interval at different times of the day; this led to the finding that greening was fastest at the time when the maximal levels of mRNA for LHC II were also observed. Whereas accumulation of chlorophyll a during a 4-h period of illumination oscillated by a factor of 3, chlorophyll b accumulation changed 10- to 15-fold. Similarly, accumulation of LHC II was highest when pigments accumulated maximally. Hence, greening or, in other words, thylakoid membrane assembly is under control of the circadian oscillator.
Keyword	[ Hide all but 1 of 17 ] b-binding-protein chlorophyll chlorophyll-a circadian-rhythm gene expression genetic regulation greening heat-shock heat-shock-proteins hordeum vulgare messenger rna plant proteins plastocyanins ribulose-bisphosphate-carboxylase structural genes thionin ubiquitin

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