Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: PNA-99-1724

[Submit comment/correction]

Reference	PNA-99-1724
Title	Enhanced ADP-glucose pyrophosphorylase activity in wheat endosperm increases seed yield
Journal	Proceedings of the National Academy of Sciences, USA
Year	2002
Volume	99
Pages	1724-1729
Author	Smidansky ED [ Show all 7 ]
Abstract	Summary: Yield in cereals is a function of seed number and weight; both parameters are largely controlled by seed sink strength. The allosteric enzyme ADP-glucose pyrophosphorylase (AGP) plays a key role in regulating starch biosynthesis in cereal seeds and is likely the most important determinant of seed sink strength. Plant AGPs are heterotetrameric, consisting of two large and two small subunits. We transformed wheat (Triticum aestivum L.) with a modified form of the maize (Zea mays L.) Shrunken2 gene (Sh2r6hs), which encodes an altered AGP large subunit. The altered large subunit gives rise to a maize AGP heterotetramer with decreased sensitivity to its negative allosteric effector, orthophosphate, and more stable interactions between large and small subunits. The Sh2r6hs transgene was still functional after five generations in wheat. Developing seeds from Sh2r6hs transgenic wheat exhibited increased AGP activity in the presence of a range of orthophosphate concentrations in vitro. Transgenic Sh2r6hs wheat lines produced on average 38% more seed weight per plant. Total plant biomass was increased by 37% in Sh2r6hs plants. Results indicate increased availability and utilization of resources in response to enhanced seed sink strength, increasing seed yield, and total plant biomass
External Databases	Pubmed: 11830676
Keyword	[ Hide all but 1 of 46 ] adp glucose pyrophosphorylase assimilate availability biomass biosynthesis brittle-2 cereal seeds determinant developing seeds dwarf spring wheat encode endosperm enzyme function gene gene expression generation heat-stress in vitro increase kernel number large subunit maize parameters pyrophosphorylase seed seed weight seed yield sensitivity shrunken-2 sink small subunit starch starch biosynthesis subunit transgene transgenic transgenic wheat triticum triticum aestivum vitro washington wheat endosperm yield zea zea mays

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.