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GrainGenes Reference Report: PNA-92-9245

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Reference	PNA-92-9245
Title	Molecular cloning and sequence analysis of expansins--a highly conserved, multigene family of proteins that mediate cell wall extension in plants
Journal	Proceedings of the National Academy of Sciences, USA
Year	1995
Volume	92
Pages	9245-9249
Author	Shcherban T [ Show all 7 ]
Abstract	Expansins are unusual proteins discovered by virtue of their ability to mediate cell wall extension in plants We identified cDNA clones for two cucumber expansins on the basis of peptide sequences of proteins purified from cucumber hypocotyls The expansin cDNAs encode related proteins with signal peptides predicted to direct protein secretion to the cell wall Northern blot analysis showed moderate transcript abundance in the growing region of the hypocotyl and no detectable transcripts in the nongrowing region Rice and Arabidopsis expansin cDNAs were identified from collections of anonymous cDNAs (expressed sequence tags) Sequence comparisons indicate at least four distinct expansin cDNAs in rice and at least six in Arabidopsis Expansins are highly conserved in size and sequence (60-87% amino acid sequence identity and 75-95% similarity between any pairwise comparison), and phylogenetic trees indicate that this multigene family formed before the evolutionary divergence of monocotyledons and dicotyledons Sequence and motif analyses show no similarities to known functional domains that might account for expansin action on wall extension. A series of highly conserved tryptophans may function in expansin binding to cellulose or other glycans. The high conservation of this multigene family indicates that the mechanism by which expansins promote wall extension tolerates little variation in protein structure.
Keyword	[ Hide all but 1 of 17 ] amino-acid-sequences arabidopsis thaliana binding-proteins cell-growth cell-wall-components cellulose-binding-proteins complementary dna cucumis-sativus gene-expression messenger-rna molecular sequence data multigene-families nucleotide-sequences oryza sativa phylogeny pisum sativum plant protein

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