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GrainGenes Reference Report: PNA-92-4138

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Reference	PNA-92-4138
Title	Involvement of small GTP-binding proteins in defense signal-transduction pathways of higher plants
Journal	Proceedings of the National Academy of Sciences, USA
Year	1995
Volume	92
Pages	4138-4144
Author	Sano H Ohashi Y
Abstract	Small GTP-binding proteins play a critical role in the regulation of a range of cellular processes--including growth, differentiation, and intracellular transportation Previously, we isolated a gene, rgp1, encoding a small GTP-binding protein, by differential screening of a rice cDNA library with probe DNAs from rice tissues treated with or without 5-azacytidine, a powerful inhibitor of DNA methylation To determine the physiological role of rgp1, the coding region was introduced into tobacco plants Transformants, with rgp1 in either sense or antisense orientations, showed distinct phenotypic changes with reduced apical dominance, dwarfism, and abnormal flower development These abnormal phenotypes appeared to be associated with the higher levels of endogenous cytokinins that were 6-fold those of wild-type plants. In addition, the transgenic plants produced salicylic acid and salicylic acid-beta-glucoside in an unusual response to wounding, thus conferring increased resistance to tobacco mosaic virus infection. In normal plants, the wound- and pathogen-induced signal-transduction pathways are considered to function independently. However, the wound induction of salicylic acid in the transgenic plants suggests that expression of rgp1 somehow interfered with the normal signaling pathways and resulted in cross-signaling between these distinct transduction systems. The results imply that the defense signal-transduction system consists of a complicated and finely tuned network of several regulatory factors, including cytokinins, salicylic acid, and small GTP-binding proteins.
Keyword	[ Hide all but 1 of 16 ] binding protein cytokinin defense-mechanisms glucosides guanosine-triphosphate mechanical-damage nicotiana tabacum oryza sativa pathogenesis-related-proteins plant pathogens plant proteins rgp1 gene salicylic-acid salicylic-acid-beta-glucoside structural genes transgenic-plants

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