Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: PNA-88-3691

[Submit comment/correction]

Reference	PNA-88-3691
Title	Restriction fragment length polymorphism-mediated targeting of the ml-o resistance locus in barley Hordeum vulgare
Journal	Proceedings of the National Academy of Sciences, USA
Year	1991
Volume	88
Pages	3691-3695
Author	Hinze K Thompson RD Ritter E Salamini F Schulze-Lefert P
Language	English
Abstract	The ml-o locus in barley confers resistance to all known races of the fungus Erysiphe garminis f. sp. hordei. Since the molecular mechanisms underlying ml-o-mediated resistance are currently undefined, experiments have been initiated to isolate the gene by means of its map position. A collection of backcross lines containing ml-o alleles derived from six barley genotypes allowed us to identify a set of DNA markers very tightly linked to the resistance locus. These markers span an unexpectedly small segment of 8.6 centimorgans on chromosome 4 that includes the Ml-o locus. Two of the markers consegregate with the resistance locus on the basis of 44 homozygous resistant plants identified within a segregating F2 population derived from an intravarietal cross. Colinearity of the resistance-linked markers was confirmed in an F2 mapping population derived from a wide cross between Hordeum vulgare subsp. vulgare and Hordeum vulgare subsp. spontaneum. The two markers consegregating with the resistance locus in the former cross define in the latter cross an interval of 2.4 centimorgans with which Ml-o is most probably situated. The set of linked markers opens up the possibility of carrying out a bidirectional chromosomal walk or jump of the gene.
Probe	bBE54 [ Show all 6 ]
Keyword	[ Hide all but 1 of 12 ] Crosses Dna Drag Erysiphe graminis f sp hordei Fungal Gene Genotype Hordeum vulgare ssp spontaneum Hordeum vulgare ssp vulgare Intravarietal Linkage Resistance

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.