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GrainGenes Reference Report: PMP-61-31

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Reference	PMP-61-31
Title	Molecular cloning and characterization of a rice phosphoinositide-specific phospholipase C gene, OsPI-PLC1, that is activated in systemic acquired resistance
Journal	Physiological and Molecular Plant Pathology
Year	2002
Volume	61
Pages	31-40
Author	Song F Goodman RM
Abstract	Summary: Phospholipid signaling is an important component in eukaryotic signal transduction pathways; in plants, it plays a key role in growth and development as well as in responses to environmental stress. We cloned and characterized a gene from rice encoding phosphoinositide-specific phospholipase C (OsPI-PLC1, Oryza sativa L. phosphoinositide-specific phospholipase C1). OsPI-PLC1 encodes a 599 amino acid protein containing the catalytic X and Y domains, as well as a C2-like domain, characteristics of this class of enzymes. Expression of OsPI-PLC1 was induced by various chemical and biological inducers of plant defence pathways, including benzothiadiazole (BTH), salicylic acid (SA), dichloroisonicotinic acid, probenazole (PB), jasmonic acid (JA) and its methyl ester, Pseudomonas syringae pv. syringae, and wounding. All of these treatments were shown to induce resistance in rice against blast disease caused by Magnaporthe grisea. OsPI-PLC1 was activated within 6 h after inoculation with the blast fungus in BTH-treated rice seedlings and in the incompatible interaction between a resistant genotype of rice and M. grisea, whereas the expression in the BTH-untreated seedlings and in the compatible interaction was only induced to a relatively low level at later time points (30 h) after inoculation, indicating that expression of OsPI-PLC1 is associated with the resistance response and/or the incompatible interaction. In addition, BTH treatment also induced systemic expression of OsPI-PLC1. These results suggest that OsPI-PLC1 may be involved in the signaling pathways leading to disease resistance in rice
Keyword	[ Hide all but 1 of 80 ] acid acquired-resistance addition amino acid amino acid sequences azoles benzothiadiazole blast blast disease blast fungus c1 cloning component defense mechanisms development disease disease resistance disease-resistance domain encodes environmental stress enzyme expression fungus gene genotype grisea growth growth and development incompatible interaction induced resistance inducers inoculation interaction jasmonic acid level magnaporthe magnaporthe grisea mechanical damage molecular molecular cloning nucleotide sequences organic acids and salts oryza oryza sativa pathway phospholipase c phospholipids plant plant defence plant diseases and disorders plant pathogenic bacteria plant pathogenic fungi plants probenazole protein pseudomonas pseudomonas syringae resistance resistant responses rice rice seedlings sa salicylic acid sativa sativum seedlings signal signal transduction signaling signaling pathway stress syringae systemic acquired resistance thiazoles time transduction transduction pathways treatment

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