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GrainGenes Reference Report: PMP-60-197

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Reference	PMP-60-197
Title	Analysis of the expression patterns of the Arabidopsis thaliana tubulin-1 and Zea mays ubiquitin-1 promoters in rice plants in association with nematode infection
Journal	Physiological and Molecular Plant Pathology
Year	2002
Volume	60
Pages	197-205
Author	Green J [ Show all 6 ]
Abstract	Summary: The temporal and spatial expression of three promoters was investigated in transgenic rice plants using promoter-beta-glucuronidase (gusA) reporter gene fusions. The promoters studied were ubiquitin-1 (UBI-1) of Zea mays, Cauliflower Mosaic Virus 35S gene (CaMV35S) and a tubulin gene (TUB-1) of Arabidopsis thaliana. The TUB-1 promoter provided 7.32-fold more GUS activity in roots relative to tillers. This was significantly different from the corresponding value of 2.82-fold for CaMV35S but not from that of 4.55-fold for UBI-1, activity of both promoters was higher in the root tips and zone of elongation than mature roots. This younger root tissue represented a declining proportion of the expanding root system with time. Older tissue expressing GUS under control of the TUB-1 promoter showed a steeper decline in activity with time than occurred with the UBI-1 promoter. Nematode infection did not alter the overall pattern of expression from the two promoters, except that the giant cells induced by Meloidogyne incognita retained TUB-1 promoter activity as roots matured. Pratylenchus zeae invaded older root regions than M. incognita and no changes in promoter activity were detected where it fed. The results suggest the TUB-1 promoter has characteristics that favour its use for delivering anti-feedants, such as cysteine proteinase inhibitors, to M. incognita
Keyword	[ Hide all but 1 of 38 ] arabidopsis beta glucuronidase cauliflower cell control cysteine cysteine proteinase inhibitor elongation fusion fusions gene gene expression infection meloidogyne meloidogyne incognita mosaic virus oryza sativa pratylenchus zeae promoter proteinase region reporter rice rice plants root root systems root tips root tissue system tissue transgenic transgenic plant transgenic rice transgenic rice plants tubulin virus zea zone

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