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GrainGenes Reference Report: PMP-59-235

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Reference	PMP-59-235
Title	Laccase isozymes: production by an opportunistic pathogen, a Fusarium proliferatum isolate from wheat
Journal	Physiological and Molecular Plant Pathology
Year	2001
Volume	59
Pages	235-242
Author	Kwon SI Anderson AJ
Abstract	Summary: Laccases often are present in plant tissues colonized by fungal pathogens. Non-denaturing gel electrophoresis revealed laccase isozymes in extracts of wheat leaf sheaths infected by Fusarium proliferatum (Fp). No laccase isozymes were detected in extracts of healthy wheat. The four anionic isozymes (LacA-A to A-D) in the infected wheat extracts corresponded to those produced by the Fp isolate when cultured on low carbon medium. Only two of these isozymes (LacA-C and A-D) were found in the culture filtrates. A single anionic laccase isozyme (LacA-B) was detected on nitrogen-limited medium. Other cationic isozymes produced in vitro were not detected from the diseased sheath tissue. Northern hybridization revealed expression of a transcript corresponding to a potential laccase-gene sequence LacRT-1 in planta and in vitro under conditions of low carbon and nitrogen supply
Keyword	[ Hide all but 1 of 49 ] alcohol biosynthesis botrytis cinerea carbon carbon- and nitrogen-starvation cryphonectria parasitica cucurbitacins culture culture filtrates electrophoresis enzymes expression extracts filtrate fungal fungal pathogen fungal pathogens fungus fusarium fusarium proliferatum gel electrophoresis healthy hybridization in vitro inducer isolate isozyme laccase laccase-encoding genes leaf leaf sheaths lignin degradation media nitrogen nitrogen supply northern northern hybridization oxidase pathogen plant tissues production proliferatum sequence single tissue transcript vitro wheat wheat leaves

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