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GrainGenes Reference Report: PMP-58-173

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Reference	PMP-58-173
Title	A modified Rpl3 gene from rice confers tolerance of the Fusarium graminearum mycotoxin deoxynivalenol to transgenic tobacco
Journal	Physiological and Molecular Plant Pathology
Year	2001
Volume	58
Pages	173-181
Author	Harris LJ Gleddie SC
Abstract	Summary: The mycotoxin deoxynivalenol (DON) produced by Fusarium graminearum is a potent inhibitor of eukaryotic protein synthesis and is believed to play a role in fungal pathogenesis on cereal crops worldwide. The putative site of action of this molecule has been theorized to be the 60S ribosomal protein L3 (RPL3). We have modified a rice (Oryza sativa L.) cDNA encoding the ribosomal protein RPL3 so that amino acid residue 258 is changed from tryptophan to cysteine, a change which is believed to confer resistance to similar mycotoxins in yeast. Both versions of the rice Rpl3 genes were introduced into two species of tobacco by Agrobacterium tumefaciens co-cultivation and expressed under the control of a constitutive promoter. When cells, tissues, and protoplasts of these transgenic tobacco plants were compared for growth in the presence of DON, a significant difference in growth rate and the ability to undergo differentiation was observed among those plants expressing the modified version of the Rpl3 gene (Rpl3:c258), compared to those expressing the wild-type Rpl3 gene. These results indicate a possible mechanism of host plant resistance to the fungal pathogen F. graminearum among the susceptible cereal species based on the expression of modified Rpl3 genes
Keyword	[ Hide all but 1 of 73 ] acid agrobacteria agrobacterium agrobacterium tumefaciens amino acid antibiotic-resistance cdna cell cell suspension cultures cereal cereal crops crop cysteine deoxynivalenol differentiation fungal fungal pathogen fusarium fusarium graminearum fusarium head blight gene gibberella ear rot graminearum growth growth rate host host plant host plant resistance inhibitor mechanism molecule mutant mycotoxin mycotoxins n-debneyi nicotiana debneyi nicotiana tabacum oryza oryza saliva pathogen pathogenesis plant resistance plant science potent promoter protein protein synthesis protoplast rate residue resistance ribosomal protein ribosomal protein l3 rice saccharomyces cerevisiae saliva sequence site synthesis tissue tobacco tobacco plants tolerance transformation transgenic transgenic tobacco transgenic tobacco plants trichodermin resistance trichothecene tolerance virulence wild-type worldwide yeast

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