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GrainGenes Reference Report: PMP-47-185

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Reference	PMP-47-185
Title	Quantitative field resistance of wheat to powdery mildew and defense reactions at the seedling stage: identification of a potential marker
Journal	Physiology and Molecular Plant Pathology
Year	1995
Volume	47
Pages	185-199
Author	Kmecl A Mauch F Winzeler M Dudler R
Abstract	Quantitative field resistance of winter wheat (Triticum aestivum L. to powdery mildew (Erysiphe graminis DC f sp tritici EM) is an important trait that is selected for by plant breeders Due to the necessary assessment in the field, the estimation of quantitative field resistance is very expensive and time-consuming Therefore, the availability of easily scorable markers for this trait would be of great advantage in wheat breeding In this study, host-responses that can be scored on individual plants at the seedling stage were assessed to see if they could serve as markers for quantitative resistance in the field. The accumulation characteristics of five defence-related gene transcripts and the formation of papillae and cell wall appositions following an infection by powdery mildew were compared in six wheat lines and one spelt (Triticum spelta L.). The lines represented a broad spectrum of different degrees of quantitative field resistance but carried no effective major resistance gene against powdery mildew. No clear correlation was found between the kinetics of levels of accumulation of defence-related gene transcripts following infection at the seedling stage and the degree of quantitative field resistance. However, cytological analysis showed that the proportion of adaxial epidermal cells that responded to pathogen attack by the formation of papillae or cell wall appositions appeared to be an indicator of high quantitative field resistance. In lines exhibiting a high quantitative field resistance, more than 80% of attacked adaxial cells responded, while in the more susceptible lines, this value dropped to less than 20%. Thus, the easily scorable proportion of attacked cells that show no. cytologically visible host-response may provide a valuable marker to predict the degree of quantitative field resistance.
Keyword	[ Hide all but 1 of 19 ] cell-structure cell-wall-appositions cytology cytopathogenicity disease-resistance epidermis erysiphe graminis-f fungal diseases gene expression leaves marker messenger rna papillae quantitative-resistance quantitative-techniques quantitative-traits tritici triticum aestivum triticum spelta

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