GrainGenes Reference Report: PMB-69-33
Reference
PMB-69-33
Title
Cloning and functional analysis of wheat V-H(+)-ATPase subunit genes.
Journal
Plant Molecular Biology
Year
2009
Volume
69
Pages
33-46
Author
[ Hide all but 1 of 7 ] Zhao Q Zhao YJ Zhao BC Ge RC Li M Shen YZ Huang ZJ
Abstract
The root microsomal proteomes of salt-tolerant and salt-sensitive wheat lines under salt stress were analyzed by two-dimensional electrophoresis and mass spectrum. A wheat V-H(+)-ATPase E subunit protein was obtained whose expression was enhanced by salt stress. In silicon cloning identified the full-length cDNA sequences of nine subunits and partial cDNA sequences of two subunits of wheat V-H(+)-ATPase. The expression profiles of these V-H(+)-ATPase subunits in roots and leaves of both salt-tolerant and salt-sensitive wheat lines under salt and abscisic acid (ABA) stress were analyzed. The results indicate that the coordinated enhancement of the expression of V-H(+)-ATPase subunits under salt and ABA stress is an important factor determining improved salt tolerance in wheat. The expression of these subunits was tissue-specific. Overexpression of the E subunit by transgenic Arabidopsis thaliana was able to enhance seed germination, root growth and adult seedling growth under salt stress.
External Databases
http://dx.doi.org/10.1007/s11103-008-9403-8
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: PMB-69-33
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||