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GrainGenes Reference Report: PMB-53-771

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Reference	PMB-53-771
Title	Identification and analysis of expressed resistance gene sequences in wheat
Journal	Plant Molecular Biology
Year	2003
Volume	53
Pages	771-787
Author	Dilbirligi M Gill KS
Abstract	Summary: Forty-eight resistance (R) genes conferring resistance to various types of pests have been cloned from 12 plant species. Irrespective of the host or the pest type, most R genes share a strong protein sequence similarity especially for domains and motifs. The objective of this study was to identify expressed R genes of wheat, the fraction of which is expected to be very low in the genome. Using modified RNA fingerprinting and data mining approaches we identified 220 expressed R-gene candidates. Of these, 125 sequences structurally resembled known R genes. In addition to 25-87% protein sequence similarity with the known R genes, the sequence, order, and distribution of the domains and motifs were also the same. Among the remaining 95, 17 were probable R-related, 21 were a new class of nucleotide-binding kinases, 21 were probable kinases, and 36 were p-loop-containing unknown sequences. About 76% were rare including 73 novel sequences. Three new R-gene specific motifs were also identified. Physical mapping of the 164 best R-gene candidates on 339 deletion lines localized 121 mappable R-gene candidates to 26 small chromosomal regions encompassing about 16% of the genome. About 90 of the 110 phenotypically characterized wheat R genes corresponding to 18 different pests also mapped in these regions
External Databases	Pubmed: 15082925
Keyword	[ Hide all but 1 of 32 ] arabidopsis candidates conferring resistance data mining disease resistance evolution expressed resistance genes family fingerprinting fraction gene gene sequence genome host identify maize mapping nb-lrr nematode resistance nucleotide binding nucleotide binding site pathogen resistance pest physical mapping protein r gene resistance resistance gene rich regions sequence specific tomato

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