Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: PMB-47-739

[Submit comment/correction]

Reference	PMB-47-739
Title	Differential expression of putative cell death regulator genes in near-isogenic, resistant and susceptible barley lines during interaction with the powdery mildew fungus
Journal	Plant Molecular Biology
Year	2001
Volume	47
Pages	739-748
Author	Huckelhoven R Dechert C Trujillo M Kogel KH
Abstract	Summary: We analysed pathogenesis-related expression of genes, that are assumed to be involved in ubiquitous plant defence mechanisms like the oxidative burst, the hypersensitive cell death reaction (HR) and formation of localized cell wall appositions (papillae). We carried out comparative northern blot and RT PCR studies with near-isogenic barley (Hordeum vulgare L. cv. Papas) lines (NILs) resistant or susceptible to the powdery mildew fungus race A6 (Blumeria graminis f.sp. hordei, BghA6). The NILs carrying one of the R-genes Mla12, Mlg or the mlo mutant allele mlo5 arrest fungal development by cell wall appositions (mlo5) or a HR (Mla12) or both (Mlg). Expression of an aspartate protease gene, an ascorbate peroxidase gene and a newly identified cysteine protease gene was upregulated after inoculation with BghA6, whereas the constitutive expression-level of a BAS gene, that encodes an alkyl hydroperoxide reductase, was reduced. Expression of a newly identified barley homologue of a mammalian cell death regulator, Bax inhibitor 1, was enhanced after powdery mildew inoculation. An oxalate oxidase-like protein was stronger expressed in NILS expressing penetration resistance. A so far unknown gene that putatively encodes the large subunit of a superoxide generating NADPH oxidases was constitutively expressed in barley leaves and its expression pattern did not change after inoculation. A newly identified barley Rac1 homologue was expressed constitutively, such as the functionally linked NADPH oxidase gene. Gene expression patterns are discussed with regard to defence mechanisms and signal transduction
Keyword	[ Hide all but 1 of 48 ] ascorbate peroxidase aspartic proteinases barley blumeria graminis cell cell death cysteine cysteine proteinases defense mechanisms development differential expression disease resistance encodes erysiphe graminis f expression fungal diseases fungus gene gene expression genes hordei hordeum hordeum vulgare inhibitor leaf line differences lines mechanism messenger rna metabolic inhibitors mildew nadph nadph oxidase oxidative burst oxidoreductases pattern pcr penetration resistance peroxidase plant powdery mildew powdery mildew fungus protein reductase resistance resistant rt-pcr signal transduction

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.