Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: PMB-45-723

[Submit comment/correction]

Reference	PMB-45-723
Title	alpha-Tubulin genes are differentially expressed during leaf cell development in barley (Hordeum vulgare L.)
Journal	Plant Molecular Biology
Year	2001
Volume	45
Pages	723-730
Author	Schroder J Stenger H Wernicke W
Abstract	Summary: Intricate changes in the patterns of the cytoskeleton, especially of microtubules, appear to control the establishment of complex plant cell shapes. Little is known about how these changes are accomplished. The objective of the present study was to test whether or not alpha-tubulin genes are differentially expressed during cell shaping in growing leaves of barley (Hordeum vulgare L.). Five alpha-tubulin genes representing at least most members of the gene family were found to be expressed in the leaf. Dot-blot analyses revealed expression patterns that could be classified into three groups. Two isotypes (HVATUB2 and HVATUB4) were maximally expressed in the meristem with a steady decline during the differentiation process (1). One isotype (HVATUB3) appeared to be constitutively expressed during cell shaping, although strongest signals were found during late stages, before the general decline in microtubular activity (2). The most striking finding was that two types (HVATUB1 and HVATUB5) were almost exclusively expressed in early post-mitotic cells, when transverse microtubular bundles determining the future cell shape in the mesophyll are formed (3). Relative transcript abundance was highest in HVATUB2 and HVATUB3, whereas the transcript level of the only transiently expressed HVATUB5 was very low, even during its phase of maximum expression. The results are discussed in the context of the general debate relating to the significance of multiple tubulin isotypes
Keyword	[ Hide all but 1 of 39 ] alpha alpha-tubulin alpha-tubulin genes arabidopsis contains barley cell cell shape cell shaping complexes cytoskeleton developing mesophyll development differential gene expression differentiation establishment expression patterns family gene gene family hordeum hordeum vulgare maximum member meristem mesophyll microtubular arrays microtubules multiple pattern phase plant science shape signal small genome transcript transcript abundance triticum aestivum tubulin zea mays l

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.