Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: PMB-45-599

[Submit comment/correction]

Reference	PMB-45-599
Title	A constitutive cystatin-encoding gene from barley (Icy) responds differentially to abiotic stimuli
Journal	Plant Molecular Biology
Year	2001
Volume	45
Pages	599-608
Author	Gaddour K [ Show all 6 ]
Abstract	Summary: A barley cDNA clone encoding a cysteine proteinase inhibitor was characterized. The deduced amino acid sequence of this barley cystatin (Hv-CPI) contains the motif QXVXG conserved among members of the cystatin superfamily. The gene (Icy), located on chromosome 2, was expressed in embryos, developing endosperms, leaves and roots as assessed by northern blot analysis. Western blot analysis detected a slightly retarded band in leaves that was not present in roots or seeds. In these two organs a more precise location of Hv-CPI was done by immuno-histochemical analysis, with polyclonal antibodies raised against the recombinant CPI protein expressed in Escherichia coli. This protein efficiently inhibited papain (Ki 2.0 x 10(-8) M) and ficin (Ki 2.2 x 10(-8) M) and, to a lesser extent, chymopapain (Ki 1.6 x 10(-7) M) and was inactive against bromelain. The Icy mRNA expression in vegetative tissues increased in response to anaerobiosis, dark and cold shock (6 degrees C)
Keyword	[ Hide all but 1 of 46 ] abiotic stress amino acid amino acid sequence animal cystatins antibody barley barley cystatin expression cdna cdna clone chromosome chymopapain chymopapain inhibitor clone cold cold shock cystatin cystatin superfamily cysteine cysteine proteinase cysteine proteinase inhibitor dark embryo endosperm escherichia coli ficin inhibitor gene inhibitor member methyl jasmonate molecular cloning mrna northern oryza ystatin papain papain inhibitor proteinase proteinase inhibitor purification rice seeds root seed sequence superfamily tissue vegetative tissues western blot analysis

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.