Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: PMB-42-615

[Submit comment/correction]

Reference	PMB-42-615
Title	Intron-mediated gusA expression in tritordeum and wheat resulting from particle bombardment
Journal	Plant Molecular Biology
Year	2000
Volume	42
Pages	615-622
Author	Salgueiro S Pignocchi C Parry MAJ
Abstract	Summary: The promoterless maize ubiquitin first exon and intron fragment can drive gusA expressed in immature tritordeum inflorescences and immature wheat scutella. In fluorescence assays, this fragment induces gusA expression in tritordeum inflorescences to 50 times higher than background. The activity of the complete promoter, exon and intron cassette was up to 20000-fold higher than background but the maize ubiquitin promoter in isolation had very low activity. A construct with the maize alcohol dehydrogenase first exon and intron had low activity, visible in histochemical assays. Both intron sequences have promoter-like features and in the ubiquitin intron there is a sequence homologous to the opaque-2-binding box. We suggest that the combination of these elements may explain the promoter activity detected in these introns
Keyword	[ Hide all but 1 of 22 ] alcohol-dehydrogenase animal assay bombardment box dna elements features fluorescence gene expression intron maize maize cells maize ubiquitin promoter particle bombardment promoter reporter gene sequence tissue transformation tritordeum ubiquitin

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.