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GrainGenes Reference Report: PMB-42-583

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Reference	PMB-42-583
Title	Cereal crops as viable production and storage systems for pharmaceutical scFv antibodies
Journal	Plant Molecular Biology
Year	2000
Volume	42
Pages	583-590
Author	Stoger E [ Show all 11 ]
Abstract	Summary: This report describes the stable expression of a medically important antibody in the staple cereal crops rice and wheat. We successfully expressed a single-chain Fv antibody (ScFvt84.66) against carcinoembryonic antigen (CEA), a well characterized tumor-associated marker antigen. scFv constructs were engineered for recombinant antibody targeting to the plant cell apoplast and ER. Up to 30 microgram/g of functional recombinant antibody was detected in the leaves and seeds of wheat and rice. We confirmed that transgenic dry seeds could be stored for at least five months at room temperature, without significant loss of the amount of activity of scFvT84.66. Our results represent the first transition form model plant expression systems, such as tobacco and Arabidopsis, to widely cultivated cereal crops, such as rice and wheat, for expression of an antibody molecule that has already shown efficacy in clinical applications. Thus, we have established that molecular pharming in cereals can be a viable production system for such high-value pharmaceutical macromolecules. Our findings provide a strong foundation for exploiting alternative uses of cereal crops both in industrialized and developing countries
Keyword	[ Hide all but 1 of 20 ] animal antibody arabidopsis thaliana bombardment carcinoembryonic antigen chain fv-antibody marker model molecular pharming nicotiana tabacum particle bombardment rice seed single-chain fv fragment storage temperature tobacco transgenic transgenic cereals transgenic tobacco

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