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GrainGenes Reference Report: PMB-40-1019

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Reference	PMB-40-1019
Title	A starch-branching enzyme gene in wheat produces alternatively spliced transcripts
Journal	Plant Molecular Biology
Year	1999
Volume	40
Pages	1019-1030
Author	Baga M Glaze S Mallard CS Chibbar RN
Abstract	Summary: A wheat gene, denoted Sbe1, encoding a type I starch-branching enzyme (SBEI) was isolated from a genomic library and shown to comprise 14 exons distributed over a 5.7 kb DNA region. Analyses of kernel RNA by 5' rapid amplification of cDNA ends (5'-RACE) and reverse transcription-polymerase chain reaction (RT-PCR) demonstrated a considerable sequence variation at the 5' ends of SBEI gene transcripts. DNA sequence alignments between the 5'-RACE products and the Sbe1 genomic DNA indicated that the first two exons and first intron were differentially processed to generate three classes of the mature transcript. One form of the SBEI gene transcript in 12-day old kernels contained the exon I + II + III combination at the 5' end, whereas other forms differed by inclusion of intron 1 or exclusion of exon II sequences. RT-PCR analysis of Sbe1-uidA::nptII chimeric mRNA produced in transgenic wheat cultured cells confirmed that the isolated Sbe1 was able to produce all three forms of SBEI gene transcripts by alternative splicing of the primary mRNA. The variants of processed Sbe1 mRNA were potentially translated into N-terminal variants of the SBEI precursor with different transit peptide sequences
Keyword	[ Hide all but 1 of 51 ] 1,4-alpha-glucan branching enzyme alternative splicing amino acid sequence amplification base sequence beta glucuronidase biotechnology cdna cytology dna dna plant dna sequence endosperm enzyme enzyme precursors enzymology exons gene gene expression genetic transformation genomic dna genomic library hexosyltransferases histoenzymology intron introns isolation and purification kernels kinases library messenger rna metabolism molecular sequence data mrna peptide promoters recombinant dna recombinant fusion proteins sequence sequence alignment sequence analysis,dna signal peptide structural genes structural plant genes tissue distribution transgenic transgenic plant transgenic wheat triticum aestivum variant variation (genetics)

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