Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: PMB-39-539

[Submit comment/correction]

Reference	PMB-39-539
Title	Regulation by biotic and abiotic stress of a wheat germin gene encoding oxalate oxidase, a H2O2-producing enzyme.
Journal	Plant Molecular Biology
Year	1999
Volume	39
Pages	539-549
Author	Berna A Bernier F
Abstract	Germins and germin-like proteins (GLPs) constitute a ubiquitous family of plant proteins that seem to be involved in many developmental and stress-related processes. Wheat germin has been extensively studied at the biochemical level: it is found in the apoplast and the cytoplasm of germinating embryo cells and it has oxalate oxidase activity (EC 1.2.3.4). Germin synthesis can also be induced in adult wheat leaves by auxins and by a fungal pathogen but it remains to be determined whether the same gene is involved in developmental, hormonal and stress response. In this work, we have studied the expression of one of the wheat germin genes, named gf-2.8, in wheat as well as in transgenic tobacco plants transformed with either this intact gene or constructs with GUS driven by its promoter. This has allowed us to demonstrate that expression of this single gene is both developmentally and pathogen-regulated. In addition, we show that expression of the wheat gf-2.8 germin gene is also stimulated by some abiotic stresses, especially the heavy metal ions Cd2+, Cu2+ and Co2+. Several chemicals involved in stress signal transduction pathways were also tested: only polyamines were shown to stimulate expression of this gene. Because regulation of the wheat gf-2.8 germin gene is complex and because its product results in developmental and stress-related release of hydrogen peroxide in the apoplast, it is likely that it plays an important role in several aspects of plant growth and defence mechanisms.
External Databases	Agricola: 2004-10560 Pubmed: 10092181
Keyword	[ Hide all but 1 of 64 ] 2,4 d adult aldehyde oxidoreductases antioxidants ascorbic acid auxin beta glucuronidase cadmium cations,divalent cobalt copper cytoplasm drug effects embryo enzyme enzyme activity enzymology experimental infections family fungal gene gene expression genetic regulation glycoproteins growth gus heavy metals hydrogen peroxide mechanical damage mechanism metabolism metal ions nicotiana tabacum oxalic acid oxidase oxidoreductases pathogen pathogenicity pharmacology plant structural genes plant viruses promoter promoters putrescine recombinant dna reducing agents regulation reporter gene roots salinity seedlings signal signal transduction sodium chloride stress stress,mechanical tobacco tobacco mosaic tobamovirus tobacco plants transgenic transgenic plant transgenic tobacco triticum aestivum virology

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.