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GrainGenes Reference Report: BBV-59-501

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Reference	BBV-59-501
Title	Cultivar and environmental conditions affect the morphogenic ability of barley (Hordeum vulgare) scutellum-derived calli
Journal	Biologia (Bratislava)
Year	2004
Volume	59
Pages	501-504
Author	Klcova L Havrlentova M Farago J
Abstract	The morphogenic ability of immature scutella-derived cultures of ten barley cultivars (Amulet, Galan, GP, Jubilant, Kompakt, Kosan, Novum, Orbit, Perun and Zlatan) was screened. Plants were field-grown in Slovakia during two seasons (1999 and 2000). All cultivars were able to produce callus and regenerate green plants. The embryogenesis and morphogenic ability of scutella was independent of callogenesis. The best responding cultivars in the first season were Golden Promise, Kosan and Novum (52.2-57%), in the second Amulet, Orbit and Kosan (24.4-40%). Differences between cultivars were statistically significant in both experiments. Despite the same regeneration protocol, the regeneration frequencies differed significantly between experiments in the two years probably because of the variability of the environmental conditions during the two seasons. Our results are consistent with previous reports indicating genotype-dependent plant morphogenesis and the effect of environmental factors on somatic embryogenesis and regeneration of barley
Keyword	[ Hide all but 1 of 14 ] barley callus cultivar embryogenesis environmental factors genetic variation in vitro culture invitro regeneration micropropagation morphogenesis regeneration scutellum seasons somaticembryogenesis

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