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GrainGenes Reference Report: PMB-29-749

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Reference	PMB-29-749
Title	Functional analysis of linker insertions and point mutations in the alpha-Amy2/54 GA-regulated promoter
Journal	Plant Molecular Biology
Year	1995
Volume	29
Pages	749-758
Author	Tregear J Primavesi L Huttly A
Abstract	Functional analysis of a gibberellin-regulated wheat alpha-amylase promoter, alpha-Amy2/54, has indicated that three regions were essential for expression By studying the ability of mutant promoters, containing a randomly inserted 22 bp excision linker, to direct expression in oat aleurone protoplasts we have refined the positions and extents of these three cis elements and also demonstrated the presence of two additional elements By converting the linker insertions to either single base point mutations or deletions using the class IIS restriction endonuclease Bsm I we have shown that nucleotides -119 and -109 within the GARE -121 GTAACAGAGTCTGG-108 and nucleotide -152 within the proposed element -156 GATTGACTTGACC-144 are essential for high level expression from this promoter
Keyword	[ Hide all but 1 of 19 ] 54-promoter aleurone-cells alpha-amy2 alpha-amylase avena sativa beta-glucuronidase deletion gene expression induced-mutations insertional-mutagenesis molecular sequence data mutant nucleotide sequence promoters protoplast recombinant-dna reporter genes transcription triticum aestivum

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