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GrainGenes Reference Report: PMB-28-949

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Reference	PMB-28-949
Title	Activity of constitutive promoters in various species from the Liliaceae
Journal	Plant Molecular Biology
Year	1995
Volume	28
Pages	949-955
Author	Wilmink A Ven B Dons J
Abstract	In this paper we first review literature on the performance of various promoters in monocotyledonous species In general, promoters isolated from monocots show a higher activity in monocot species Moreover, the presence of an intron between the promoter and reporter gene increases transcription levels We used the same approach to study gene expression in Liliaceae. The activities of the CaMV 35S, maize Adh1-based pEmu, rice Act1 and maize Ubi promoters, coupled to the beta-glucuronidase (gus) reporter gene, were evaluated for transient gene expression upon particle bombardment of tissues of tobacco, rice, tulip, lily and leek. Although monocot promoters performed very well in rice tissues, the results of this study show that this cannot be generalized for other monocot species. The transcription inducing effects of monocot promoters were less pronounced or even absent in tissues of Liliaceae, while the presence of an intron between promoter and gus gene reduced promoter activity.
Keyword	[ Hide all but 1 of 20 ] 35s-promoter act1-promoter allium-ampeloprasum beta-glucuronidase emu-promoter gene expression genetic transformation introns lilium-longiflorum nicotiana plumbaginifolia nicotiana tabacum oryza sativa promoters recombinant-dna reporter genes transcription transgenic-plants tulipa-gesneriana ubi-promoter zea mays

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