Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: PMB-27-815

[Submit comment/correction]

Reference	PMB-27-815
Title	The effect of intracellular pH on the regulation of the Rab 16A and the alpha-amylase 1/6-4 promoter by abscisic acid and gibberella
Journal	Plant Molecular Biology
Year	1995
Volume	27
Pages	815-820
Author	Heimovaara-Diijkstra S Mundy J Wang M
Abstract	Intracellular pH (pHi) of barley aleurone cells is known to be affected by hormones and plant growth conditions The possible mechanisms by which these pHi shifts influence the actions of abscisic acid (ABA) or gibberellin (GA) is being investigated Here we report an attempt to study the effect of pHi on hormone-induced gene expression We used weak acids and weak bases to artificially mimic the pHi changes brought about by ABA and GA and found that chloramphenicol acetyltransferase (CAT) expression controlled by the Rab promoter was affected while the alpha-amylase promoter seemed insensitive CAT fused to the 35S promoter was used as a control which is not inducible by ABA or GA3 The expression of this construct was not significantly affected by artificial pHi changes.
Keyword	[ Hide all but 1 of 14 ] abscisic acid aleurone-cells alpha-amylase chloramphenicol-acetyltransferase enzyme activity gene expression genetic regulation gibberellins hordeum vulgare ph promoters rab-16a-promoter recombinant-dna reporter genes

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.