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GrainGenes Reference Report: PMB-26-1191

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Reference	PMB-26-1191
Title	Molecular cloning of the Arabidopsis thaliana sedoheptulose-1,7-bisphosphatase gene and expression studies in wheat and Arabidopsis thaliana
Journal	Plant Molecular Biology
Year	1994
Volume	26
Pages	1191-1200
Author	Willingham N Lloyd J Raines C
Abstract	We report here the isolation and nucleotide sequence of genomic clones encoding the chloroplast enzyme sedoheptulose-1,7-bisphosphatase (SBPase) from Arabidopsis thaliana The coding region of this gene contains eight exons (72-76 bp) and seven introns (75-91 bp) and encodes a polypeptide of 393 amino acids Unusually, the 5' non-coding region contains two additional AUG codons upstream of the translation initiation codon A comparison of the deduced Arabidopsis and wheat SBPase polypeptide sequences reveals 78.6%, identity Expression studies showed that the level of SBPase mRNA in Arabidopsis and wheat is regulated in a light-dependent manner and is also influenced by the developmental stage of the leaf. Although the Arabidopsis SBPase gene is present in a single copy, two hybridizing transcripts were detected in some tissues, suggesting the presence of alternate transcription start sites in the upstream region.
Keyword	[ Hide all but 1 of 14 ] amino acid sequence arabidopsis thaliana exons gene expression genetic regulation introns leaves light messenger rna molecular sequence data nucleotide sequence phosphoric-monoester-hydrolases structural genes triticum aestivum

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