GrainGenes Reference Report: PMB-26-535
Reference
PMB-26-535
Title
A chimeric gene (orf256) is expressed as protein only in cytoplasmic male-sterile lines of wheat
Journal
Plant Molecular Biology
Year
1994
Volume
26
Pages
535-539
Author
Song J Hedgcoth C
Abstract
Mitochondria derived from Triticum timopheevi have a chimeric gene, orf256, immediately upstream from coxI Antibodies to a peptide corresponding to a part of the encoded amino acid sequence of orf256 detect a 7 kDa protein on u-estern blots of mitochondrial proteins from cytoplasmic male-sterile (cms) wheat (T aestivum nucleus, T. timopheevi mitochondria) but not in mitochondrial proteins from T. aestivum, T. timopheevi, or cms plants restored to fertility by introduction of nuclear genes for fertility restoration. The 7 kDa protein appears to serve as a marker for cms wheat. Its occurrence as an integral protein of the inner membrane may indicate a cms effect through an influence on mitochondrial membrane function.
Keyword

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: PMB-26-535
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